Skip to main content
. 2017 Nov 21;9:12–22. doi: 10.1016/j.omtm.2017.11.006

Figure 4.

Figure 4

Proteolysis of lclPTENL

(A) U87MG cells expressing either PTEN-L or lclPTEN-L were treated without or with doxycycline for 48 hr. Total cell lysates were analyzed by western blot with antibody to PTEN. (B and C) U87MG cells expressing either PTEN-L (B) or lclPTEN-L (C) were treated for 24 hr with doxycycline and/or brefeldin A as indicated. Total cell lysates were analyzed by western blot using antibody to PTEN. (D) Quantitation of changes in intracellular lclPTEN-L with brefeldin A treatment. Open circles show quantitation of the high molecular weight band, and filled circles show quantitation of the low molecular weight band.