Figure 3.

RNase H1 Knockdown Suppresses LNA-ASO Toxicity In Vitro

(A) Mouse 3T3 fibroblast cells were transfected with a siRNA against RNase H1 (light gray bars) and a control (ctr.) siRNA (dark gray bars) at 10 nM, respectively, using Lipofectamine 2000. 24 hr later, cells were transfected with the hepatotoxic LNA41 or LNA37 at 30 nM using Lipofectamine 2000. Caspase 3/7 activity was measured 24 hr later. (B) siRNA transfection was performed as in (A) and cells were harvested 48 hr later for assessing RNase H1 knockdown by qPCR. Results in (A) and (B) are shown as a percentage change relative to UTCs. Data (n = 3) are mean ± SD.