Figure 7.

Higher Affinity LNA-ASOs Tend to Be More Toxic In Vitro and In Vivo

(A) The 236 LNA-ASOs were separated into two groups with in vivo mean ALT levels ≤5-fold and >5-fold from saline-treated mice, respectively. For each group, the respective theoretical T_m values were calculated and summarized in a boxplot diagram. (B) The 236 LNA-ASOs were separated into four T_m ranges, as indicated. For each T_m range, the oligonucleotides’ respective caspase values were listed in a boxplot diagram. In addition, for each group in (A) and (B) the number (count) of included LNA-ASOs, the median T_m (for A) and caspase (for B) values, and the number of outliers are shown. The level of significance as evaluated by Wilcoxon rank sum test is indicated above the boxplots in (A).