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FRET evolution of the dye-loaded host with different blank containers. (A, B) The interaction of the dye (DiI+DiO) loaded nanoassembly with the corresponding blank nanoassembly (leaky assembly and the presence of a reservoir that can accept guests). (C, D) The interaction of the dye (DiI+DiO) loaded nanogel with the blank nanogel (stably encapsulating nanogel or the lack of a reservoir to accept the guest molecule).
