Skip to main content
. 2017 Jul 17;33(6):603–615. doi: 10.1007/s12264-017-0153-y

Fig. 6.

Fig. 6

Ppargc1b is a functional target of miR-30a in microglia. A Schematic of the luciferase reporter system. B Putative miR-30a binding sites and mutations in Ppargc1b mRNA 3′ UTR. C Luciferase activity of reporter carrying the wild-type (WT) or mutated (Mut) Ppargc1b mRNA 3′ UTR co-transfected into HEK293T cells with Agomir-30a or Agomir-NC. D, F Western blots of Ppargc1b in microglia transduced with miR-30aOE (D) or miR-30ai (F) lentivirus vector. E, G Statistical analysis of Ppargc1b densitometry normalized to GAPDH in D and F (n = 3). H, I Quantitative PCR analysis of PPARγ in microglia transduced with miR-30aOE (H) or miR-30ai (I) lentivirus vector versus control (n = 3). *P < 0.05 (Student’s t test); mean ± SD.