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Crocetin increases neurite growth in cultured hippocampal neurons. A, B Representative images of cultured hippocampal neurons two days after plating without (Control) and with 10 μmol/L crocetin. Cells were immunostained with β-tubulin antibody. Crocetin (5 μmol/L–20 μmol/L) promoted growth of the longest neurite (C) in individual neurons. At least 100 neurites were analyzed in 20 fields from each group. Data are presented as mean ± SD; *P < 0.05, **P < 0.01 versus control.
