Relationship between the mosquito infection rate and Plasmodium vivax parasite, gametocyte, and pvs25 transcript densities in blood (using loop-mediated isothermal amplification). Circles represent the infection rates of individual feeding experiments. Trend lines are three parameter logistic regression; the shaded areas represent the 95% confidence intervals. (A, C, E) Experiments without human AB-serum replacement. (B, D, F) Experiments with AB malaria-naïve serum replacement. nd, not detected.