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. 2017 Dec 11;7:17346. doi: 10.1038/s41598-017-17439-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MAT1-2-1 is recruited to the cbh1 promoter upon cellulase induction. ChIP analyses of MAT1-2-1 recruitment to the −800 and −200 regions of the cbh1 promoter (A and B), the pheromone receptor gene promoters (hpr1 and hpr2) (C), and the pheromone precursor gene promoters (hpp1 and pgg1) (D) before (Gly) and after induction with Avicel for the indicated time periods. (E) Semi-quantitative PCR products amplified from the above promoter fragments in the immunoprecipitated samples. GM and WT denoted samples from the EGFP-MAT1-2-1 and TU6-RP strains, respectively. Gly denoted pre-cultures on glycerol.