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. 2017 Dec 11;7:17346. doi: 10.1038/s41598-017-17439-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MAT1-2-1 recruitment to the cbh1 promoter is dependent on Xyr1. (A) Expression and subcellular localization of EGFP-MAT1-2-1 in the Δxyr1 strain. (B and C) ChIP analyses of MAT1-2-1 recruitment to the −800 and −200 regions of the cbh1 promoter in the presence or absence of Xyr1 after induction with Avicel for the indicated time periods. Gly denoted pre-cultures on glycerol. (D) ChIP analyses of MAT1-2-1 recruitment to different regions of the cbh1 promoter under Avicel inducing conditions in the EGFP-MAT1-2-1, Δxyr1_EGFP-MAT1-2-1 strains and a control strain expressing EGFP. Error bars indicated the SD from two independent biological replicates. The actin, hpr1 and hpr2 promoters were used as controls. Significant differences (T-test *P < 0.05, **P < 0.01, ***P < 0.001, n.s. not significant) were detected for the recruitment of EGFP-MAT1-2-1 to all the tested regions of the cbh1 promoter between the EGFP-MAT1-2-1 and the Δxyr1_EGFP-MAT1-2-1 strains. No significant recruitment was detected for MAT1-2-1 to the actin and hpr1 promoters or for GFP alone to all the tested promoters.