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. 2017 Dec 7;11:662. doi: 10.3389/fnins.2017.00662

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Copyright © 2017 Beltz and Benton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Actively proliferating (BrdU+) cells in the neurogenic niche have a bimodal temporal distribution. BrdU+ cells were quantified in the niches of crayfish that were sacrificed daily for 1 week after BrdU injection, and at intervals thereafter for 21 days. The probability of observing BrdU+ cells in the niche was then plotted for each of the sampling days. BrdU+ cells were observed in the niche on days 1–4 following injection (shown in the day 1 image below graph). On days 5–7, the niches contained no BrdU+ cells (GAP). However, between days 8 and 14 after injection, BrdU+ cells were once again observed in the niche. Adapted from Benton et al. (2014). Scale bar: 50 μm.