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. 2017 Dec 11;7:17288. doi: 10.1038/s41598-017-17304-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological validation of the murine colonic explant culture system (A) Hematoxylin eosin safranin (HES) staining of a normal colon section of an uninfected SCID mouse. Scale bar, 40 µm. (B) HES staining of a colonic explant section after 14 days of culture showing good tissue preservation with a characteristic collagen fiber and crypt-like structures. Scale bar, 20 µm. (C,D) HES staining of a colonic explant section after 21 and 30 days of culture, respectively. Tissue preservation was confirmed with a presence of high prismatic epithelium, basal position of nucleus, a well-organized basal lamina and the presence of crypt-like structures (black arrow (D)). Presence of large cyst-like formations (white arrow (C)). Scale bars, 20 µm.