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. 2017 Dec 11;7:17288. doi: 10.1038/s41598-017-17304-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Development of neoplasia in the murine colonic explant model. (A) Hematoxylin eosin safranin (HES) staining of an uninfected colonic explant section showing a normal epithelial structure. Scale bar, 65 µm. (B) HES staining showing a low-grade intraepithelial lesion in a colonic explant after 27 days of infection with C. parvum (grey arrow) characterized by: (i) loss of cell-to-cell contact (ii) reduction of the interglandular space (white arrow) (iii) loss of nuclear polarity with slight pseudostratification (black arrow). Scale bar, 25 µm.