Fig. 4.

Combined loss of VHL and PBRM1 improves cellular fitness. a MEF viability at 6 days following exposure to Mitomycin C (n (independent experiments) = 4). b qPCR analysis of the recombined Vhl allele present in renal cortical cells of tamoxifen treated Vhl ^fl/fl (Vhl ^−/−) and Vhl ^fl/fl;Pbrm1 ^fl/fl (Vhl ^−/−;Pbrm1 ^−/−), (Vhl ^−/−: n = 8 mice <12 m; 5 mice >20 m. Vhl ^−/−;Pbrm1 ^−/−: n = 7 < 12 mice, 10 mice >20 m). c Representative images of CA9 immunohistochemistry of Vhl ^−/− and Vhl ^−/−;Pbrm1 ^−/− mice at indicated ages. Graphs depict mean ± s.e.m. (error bars). (a) Two-way ANOVA, Sidak’s correction. (b) Two-way ANOVA, Tukey’s correction. ****P < 0.0001; ***P < 0.001; **P < 0.01