Table 1.
Analysis of mouse abdominal aortae using elastin van Gieson staining and immunohistochemistry for actin and GSL.
| Mmp-7+/+ Fc (n = 18) | Mmp-7+/+ EC4-Fc (n = 17) | Mmp-7−/− Fc (n = 17) | Mmp-7−/− EC4-Fc (n = 19) | |
|---|---|---|---|---|
| Smooth muscle cell content | ||||
| %: actin | 95.9 ± 1.6 | 92.5 ± 3.3 | 94.3 ± 1.2 | 91.2 ± 1.7 |
| %: desmin | 77.0 ± 4.4 | 79.7 ± 3.0 | 68.0 ± 4.7 | 81.5 ± 4.4 |
| Macrophage content | ||||
| %: GSL | 0.19 ± 0.07 | 0.45 ± 0.17 | 0.41 ± 0.23 | 0.24 ± 0.12 |
| %: CD68 | 0.73 ± 0.35 | 0.61 ± 0.10 | 0.57 ± 0.15 | 0.88 ± 0.43 |
| Cellular Senescence (%: p16) | 4.01 ± 2.04 | 2.22 ± 0.59 | 2.19 ± 0.61 | 3.42 ± 1.07 |
| Cell density (cells/area) | 0.66 ± 0.05 | 0.65 ± 0.07 | 0.87 ± 0.13 | 0.57 ± 0.02 |
| Medial area (µm² × 1000) | 252 ± 77 | 280 ± 98 | 117 ± 16 | 164 ± 43 |
| Lumen diameter (µm) | 678 ± 37 | 677 ± 39 | 649 ± 38 | 630 ± 41 |
| Medial thickness (µm) | 47.2 ± 2.4 | 45.4 ± 1.9 | 43.5 ± 2.4 | 45.9 ± 2.9 |
| Elastic lamina breaks (# broken layers) | 2.2 ± 0.4 | 2.1 ± 0.5 | 1.9 ± 0.4 | 2.6 ± 0.5 |