Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 11;7:17344. doi: 10.1038/s41598-017-17337-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

RNA-Seq flowchart. The samples were processed according to the five different methods that were selected, and whose quantifications included both read counts (simply the number of reads overlapping a given feature such as a gene) and fpkm (Fragments Per Kilobase of exon per Million reads). With the latter it is possible to compare genes of different lengths, and ‘per million reads’ means that a value normalized against the library size is obtained. The consensus occurred between significant detections in both read counts and fpkm scenarios, before reaching a global result (overall consensus). The mutational profile at the right side was implemented under simplified algorithmic conditions (CuffDiff), and for two major mutations (BRAF, NRAS) (Supplementary Tables 10.1-5). The validations refer to candidates coming from all the considered scenarios.