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Western blot analysis of AT2G21280. The specificity of the antibodies and the protein level of AT2G21280 in the Col wild type, sgRNA mutants, T-DNA mutants and overexpression plant were analyzed. Total proteins were extracted from leaves of 4-week-old plants, and loaded in each lane. RbcL was used as a loading control. The molecular weight of protein markers are labeled on the right.
