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. 2017 Dec 11;7:17355. doi: 10.1038/s41598-017-17729-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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G–CSF biomarker is elevated early in FSL–1–treated mice. Male C57BL/6 mice were administered physiological water (NT) or FSL–1 at 24 hours post 9.2 Gy TBI. Peripheral blood was collected on days 3, 9, 17 and 31 post TBI. G–CSF levels were assessed by (a) luminex or (b) ELISA. (c) EPO and (d) TPO levels were assessed by ELISA. Luminex data represents one experiment, while ELISA data are cumulative of 2 independent experiments. Each symbol represents one mouse. *P < 0.05 or **P < 0.01 between TBI, NT and TBI, FSL–1–treated mice. ⁺ P < 0.05, ⁺⁺ P < 0.01 or ⁺⁺⁺⁺ P < 0.0001 between FSL–1 and TBI, FSL–1–treated mice.