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. 2017 Dec 11;7:17296. doi: 10.1038/s41598-017-17558-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expressions of inducible nitric oxide synthase (iNOS; A), phosphorylated AKT (B), IRS-1 (C) and IRβ (D) in lung tissues lean and high-fat diet-fed mice, treated or not with aminoguanidine (AG; 20 mg/kg/day, 3 weeks). Mice received an intravenous injection of insulin (INS; 1 U/animal), and within 5 min the lungs were processed. Phosphorylated AKT, IRS-1 AND IRβ and iNOS were normalized for β-actin. Data are expressed as mean ± SEM (n = 6). *p < 0.05 compared with respective basal (non-stimulated) group; ^#p < 0.05 compared with untreated obese group; **p < 0.05 compared with lean untreated mice; ^##p < 0.05 compared with obese untreated mice.