Fig. 4.

The suhB BoxA overlaps the first of multiple Rut elements. β–galactosidase activity of short (a) and full-length (b) suhB transcriptional fusions to lacZ. Constructs included either a wild-type sequence (‘wt’), boxA mutation (‘C4T, T6C’) and/or suhB start codon mutation (‘ATG → CAG’), as indicated on the x-axis. 200 bp of sequence upstream of the suhB gene was included in all constructs. Error bars represent ±1 standard deviation from the mean (n = 3). Schematics of the constructs used for these experiments are depicted below the graphs