Fig. 5.

The effect on suhB-lacZ transcription levels of altering the distance between boxA and the S-D sequence. β–galactosidase activity of wild-type (‘wt boxA’; dark grey bars) and boxA mutant (‘C4A’; blue bars) transcriptional fusions of suhB to lacZ, with increasing lengths of non-coding DNA inserted between the boxA and S-D sequences. The length of inserted sequence (nt) is indicated on the x-axis. Constructs include 200 bp of upstream sequence and a full-length suhB fused to lacZ in the pAMD-BA-lacZ plasmid. Note that the sequence of inserted non-coding DNA differs for constructs with insertion sizes of ≤32 bp and ≥40 bp (see Methods and Supplementary Fig. 6 for details)