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. 2017 Dec 11;7:17326. doi: 10.1038/s41598-017-17509-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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WNT5A promotes IL6 production in human preadipocytes in vitro. (A) Baseline mRNA expression of WNT5A and IL6 was measured in visceral/omental and subcutaneous preadipocytes by qRT-PCR (n = 5). (B) Transcript levels of IL6 after treatment with recombinant WNT5A protein (1 µg/ml for 8 hours), assessed by qRT-PCR analysis. (C,D) Transcript levels of IL6 after treatment with with siRNA against WNT5A (C) or scrambled siRNA (D) as control. Data represented as percentage of WNT5A knock-down and percentage of change in IL6 mRNA levels.