Fig. 3.

Construction of five RGEPs for benchmarking the estimation accuracy. a For each source location (melanoma, ascites, PBMC) individual single cell gene expression profiles are collected for multiple patients. Colours indicate the cell type, numbers indicate the patient sample and symbols show the source location (triangles for melanoma, squares for PBMCs, and diamonds for ascites). b Construction of REGPs from three single cell data-sets: RGEP1 bases on the population average of PBMC data; RGEP2 takes all three source locations into account; RGEP3 is indication-specific and location-specific; CNTR1 is patient-specific for tumour cells and indication/location-specific for non-malignant cells; CNTR2 is fully patient-specific