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. 2017 Dec 11;7:17358. doi: 10.1038/s41598-017-17666-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of OPN on periodontal ligament cells (PDL) proliferation. Three PDL cell lines established from three different donors were cultured on gelatin-coated (9 ng/well), commercial hOPN-coated (9 ng/well) and plant-produced hOPN-coated surface (1. 5 and 9 ng/well) for 1–3 days. The experiments were done in triplicated. Cell survival was evaluated by MTT assay at 24 hours. Data represented the absorbance at 540 nm. Data are means of 3 independent replicate samples +/−SD. Control in this experiment is the untreated cells. (*p ≤ 0.05).