Figure S1.

U251 cells were transfected with anti-miR-control/anti-miR-6743-5p, and infection with shGRIM-19 or shNC lentivirus as indicated. Cells without any treatment were set as a negative control (Mock). After 48 h, cells were fixed and subjected to TdT-mediated-dUTP nick end labeling (TUNEL) staining (Green) with In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s protocol. Cells were counterstained with DAPI (blue). The representative images (A) and the quantitative analysis (B) of TUNEL assays are shown. ***P<0.001 vs. Mock and anti-miR-control+shNC; ###P<0.001 vs. anti-miR-6743-5p+shNC; +++P<0.001 vs. anti-miR-control+shGRIM-19.

bsr20171038_Supp1.pdf ^{(318KB, pdf)}