Figure 5. Effects of H₂O₂ on the expression of mitochondrial respiratory chain complex subunits and bioenergetics metabolism of TE-1 cells.

(A) TE-1 cells were pretreated with or without 200 μM H₂O₂ for 24 h and the cells were subjected to Western blot analysis. (B) OCR of TE-1 cells treated with H₂O₂ (200 μM). (C) ATP production, basal, and maximal respiration are presented as mean ± S.D. of six replicates. (D) ECAR of TE-1 cells treated with H₂O₂ (200 μM). (E) Quantitation of the ECAR performed on TE-1 cells as shown in (B). The data are presented as mean ± S.D.; *P<0.05, ***P<0.001.