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. 2018 Jan 10;28(2):110–130. doi: 10.1089/ars.2016.6869

FIG. 10.

FIG. 10.

dRP stimulates VEGFR2 upregulation and NOX2-deoendent angiogenesis in vivo. (A) Representative examples (i) and quantification (ii) of the hematoxylin/eosin staining of wound tissue treated with dRP or vehicle control (PBS). Data represent counts of vascular structures (surrounded by continuous intimal monolayer) per optic field. Statistical significance of the difference was assessed by nonparametric Mann–Whitney test (*p < 0.05 compared with vehicle, n = 10). Vascular structures are highlighted by white arrows in the picture. (B) Representative examples (i) and quantification (ii) of the VEGFR2-specific staining of wound tissue treated with dRP or vehicle control (PBS). Data represent counts of high fluorescence intensity areas per mm2, as estimated using ImageJ. Statistical significance of the difference was assessed by nonparametric Mann–Whitney test (*p < 0.05 compared with vehicle, n = 8). (C) Representative examples (i) and quantification (ii) of hematoxylin staining of Matrigel™ plugs containing dRP (41.2 μg/plug) or vehicle control (PBS) after 7 days of implantation in wild-type (C57BL6/J) or NOX2−/− (B6.129S-Cybbtm1Din/J; Jackson Laboratories) mice. Data represent counts of capillary structures per mm2, as estimated using ImageJ. Statistical significance of the difference was assessed by one-way ANOVA with Bonferroni post hoc test (*p < 0.05 compared with vehicle, **p < 0.05 compared with dRP, n = 6). (D) Representative examples (i) and quantification (ii) of the DAPI/CD31-specific staining of Matrigel plugs containing dRP (41.2 μg/plug) or vehicle control (PBS) after 7 days of implantation in wild-type (C57BL6/J) or NOX2−/− (B6.129S-Cybbtm1Din/J; Jackson Laboratories) mice. Data represent counts of capillary structures per mm2, as estimated using ImageJ. Statistical significance of the difference was assessed by one-way ANOVA with Bonferroni post hoc test (*p < 0.05 compared with vehicle, **p < 0.05 compared with dRP, n = 6). Bars: 300 μm (throughout). To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars