Table 2.
Predicted signal peptides and cleavage sites in known plastid‐localized proteins and Type II FAS sequences in Karenia brevis. Signal peptide prediction and score generated by signalP. The D‐score (discrimination score) is used to discriminate signal peptides from non‐signal peptides, using a threshold of 0.5. Cleavage site sequences were screened for the conserved FVAP sequence found in peridinin dinoflagellates. In some sequences a plausible FVAP‐like sequences is present, but most possess the RRV(Q/K) also present in Karlodinium. No signal peptides were present in any single domain PKS KS or KR sequences
| GenBank accession no. or contig no. | CD and blast ID | Prediction | D score | Cleavage site |
|---|---|---|---|---|
| ABF73013 | Cytochrome b6f FeS subunit | No | 0.131 | – |
| Contig 15936 | Cytochrome b6‐f | Yes | 0.55 | G‐QSPREQ |
| ABF73015 | Ferredoxin | Yes | 0.613 | R‐RRVRD |
| Contig 15936 | Multimeric flavodoxin | Yes | 0.763 | A‐ASLAS |
| ABF73016 | Flavodoxin nadph reductase | Yes | 0.673 | G‐FRVQ |
| ABF73017 | Oxygen enhancer | No | 0.419 | G‐RFQQK |
| ABF73018 | psII 12kD | Yes | 0.864 | A‐FSPA |
| ABF73029 | GapDH C1 | Yes | 0.606 | A‐(+9)FEEQ |
| ABF73002 | GapDH C1 | No | 0.323 | A‐FIAPA |
| kbrevis_combined_contig_386 | KASII 3 oxoacyl ACP synthase | Yes | 0.7 | G‐RRVQ or G‐(+9)FKPA |
| kbrevis_combined_contig_387 | KASII 3 oxoacyl ACP synthase | Yes | 0.681 | G‐RRVQ or G‐(+9)FKPA |
| kbrevis_combined_contig_46229 | KASII 3 oxoacyl ACP synthase | Yes | 0.723 | S‐DYGR or G‐RRVK |
| kbrevis_combined_contig_37304 | KASII 3 oxoacyl ACP synthase | Yes | 0.607 | G‐RRVQ or A‐FNPA or G‐(+9)FNPA |
| kbrevis_combined_contig_49622 | KASIII 3 oxoacyl ACPsynthase | Yes | 0.816 | G‐RRIL |
| kbrevis_combined_contig_275 | KASII 3 oxoacyl ACP synthase | Yes | 0.572 | G‐RRLN |
| kbrevis_combined_contig_97889 | KR | Yes | 0.837 | A‐QTPT or A‐(+9)FPHA |
| kbrevis_combined_contig_64411 | KR | Yes | 0.479 | G‐RPMQ |
| kbrevis_combined_contig_86499 | KR | Yes | 0.798 | A‐YGEF or C‐SSRE |
| kbrevis_combined_contig_28593 | ER | Yes | 0.646 | G‐KRVQ |
| kbrevis_combined_contig_47965 | ER | Yes | 0.69 | G‐KRVK |
| kbrevis_combined_contig_44484 | DH | No | 0.148 | – |
| kbrevis_combined_contig_6314 | AT | Yes | 0.659 | G‐RTLQ |
| kbrevis_combined_contig_101182 | AT | No | 0.427 | G‐RRLQ |