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. 2017 Nov 8;41(12):2066–2081. doi: 10.1111/acer.13511

Figure 7.

Figure 7

Effects of chronic ethanol (EtOH) and acute stress on CD11b+ IR and c‐Fos+ IR in the prelimbic cortex (PrL). Rats were treated with chronic EtOH (5.0 g/kg, 20 to 30% v/v, 2 days on, 2 days off from P25 to P54) and/or acutely stressed with a 2‐hour restraint/water immersion stressor following prolonged abstinence on P96/P97. The rats were sacrificed 2 hours following the conclusion of the stressor. (A) CD11b+ pixel density in the PrL was assessed in each group. Data are presented as mean ± SEM. *p < 0.05 compared to CON, † p < 0.05 compared to EtOH, ‡ p < 0.05 compared to Stress (Tukey's post hoc test). n = 8 to 10/group. A representative image of CD11b staining from each group is shown. A higher magnification image is displayed in the inset. The scale bar in the low‐magnification image measures 100 microns, and the scale bar in the high‐magnification inset measures 20 microns. (B) c‐Fos+ cell density in the PrL was assessed in each group. Data are presented as mean ± SEM. *p < 0.05 compared to CON (Tukey's post hoc test). n = 8 to 10/group. A representative image of c‐Fos staining from each group is shown. A higher magnification image is displayed in the inset. The scale bar in the low‐magnification image measures 100 microns, and the scale bar in the high‐magnification inset measures 20 microns.