Table 6.
Brain region | CON | AIE | Stress | AIE+Stress |
---|---|---|---|---|
Prefrontal cortex | ||||
PrL | 1,086 ± 133a | 1,197 ± 70a | 1,424 ± 116b | 1,492 ± 63b |
IL | 701 ± 77a | 733 ± 51a | 1,008 ± 78b | 941 ± 89b |
Bed nucleus of the stria terminalis | ||||
dBNST | 115 ± 16a | 102 ± 16a | 231 ± 19b | 253 ± 26b |
vBNST | 163 ± 29a | 164 ± 23a | 335 ± 28b | 376 ± 25b |
Hypothalamus | ||||
PVN | 352 ± 90a | 217 ± 70a | 879 ± 115b | 1,017 ± 97b |
Amygdala | ||||
BLA | 271 ± 17a | 203 ± 30a | 244 ± 28a | 271 ± 22a |
CeA | 395 ± 35a | 232 ± 58b | 581 ± 52c | 413 ± 33a |
Periaqueductal gray | ||||
lPAG | 86 ± 16a | 103 ± 14a | 154 ± 18b | 158 ± 17b |
BLA, basolateral amygdala; CeA, central nucleus of the amygdala; dBNST, dorsal bed nucleus of the stria terminalis; IL, infralimbic cortex; lPAG, lateral periaqueductal gray; PVN, paraventricular nucleus of the hypothalamus; PrL, prelimbic cortex; vBNST, ventral bed nucleus of the stria terminalis.
Rats were treated with chronic intermittent EtOH and, after a period of prolonged abstinence, were acutely stressed with 2 hours of restraint/partial water immersion. Rats were sacrificed 2 hours following the termination of the stressor. Brain regions were stained for EGR1 to assess neuronal activation. n = 6 to 10 per group. Data are presented as mean ± SEM. Statistically significant differences are indicated by different letters; for example, means with superscripts “a” and “b” are statistically different (Tukey's post hoc test), while means with superscripts “a” and “a” are not statistically different.