. 2017 Nov 8;41(12):2066–2081. doi: 10.1111/acer.13511

Table 6.

Effects of Chronic Ethanol (EtOH) and Acute Stress on EGR1 Expression Across Brain Regions (cells/mm²)

Brain region	CON	AIE	Stress	AIE+Stress
Prefrontal cortex
PrL	1,086 ± 133^a	1,197 ± 70^a	1,424 ± 116^b	1,492 ± 63^b
IL	701 ± 77^a	733 ± 51^a	1,008 ± 78^b	941 ± 89^b
Bed nucleus of the stria terminalis
dBNST	115 ± 16^a	102 ± 16^a	231 ± 19^b	253 ± 26^b
vBNST	163 ± 29^a	164 ± 23^a	335 ± 28^b	376 ± 25^b
Hypothalamus
PVN	352 ± 90^a	217 ± 70^a	879 ± 115^b	1,017 ± 97^b
Amygdala
BLA	271 ± 17^a	203 ± 30^a	244 ± 28^a	271 ± 22^a
CeA	395 ± 35^a	232 ± 58^b	581 ± 52^c	413 ± 33^a
Periaqueductal gray
lPAG	86 ± 16^a	103 ± 14^a	154 ± 18^b	158 ± 17^b

BLA, basolateral amygdala; CeA, central nucleus of the amygdala; dBNST, dorsal bed nucleus of the stria terminalis; IL, infralimbic cortex; lPAG, lateral periaqueductal gray; PVN, paraventricular nucleus of the hypothalamus; PrL, prelimbic cortex; vBNST, ventral bed nucleus of the stria terminalis.

Rats were treated with chronic intermittent EtOH and, after a period of prolonged abstinence, were acutely stressed with 2 hours of restraint/partial water immersion. Rats were sacrificed 2 hours following the termination of the stressor. Brain regions were stained for EGR1 to assess neuronal activation. n = 6 to 10 per group. Data are presented as mean ± SEM. Statistically significant differences are indicated by different letters; for example, means with superscripts “a” and “b” are statistically different (Tukey's post hoc test), while means with superscripts “a” and “a” are not statistically different.