Table 3.
Yield (mol/mol [glucose]) | ||||||||
---|---|---|---|---|---|---|---|---|
Strain | Temperature/preculture condition | μ [hr−1] | r glucose [mmol/gDW/hr] | Y biomass/glucose [g/mmol] | Itaconate | Glutamate | Acetate | Pyruvate |
MG1655 | 30°C PcL,MM | 0.36 ± 0.01 | 4.36 ± 0.27 | 0.082 ± 0.003 | 0 | 0.00 ± 0.00 | 0.34 ± 0.00 | 0 ± 0 |
37°C PcL,MM | 0.71 ± 0.01 | 7.62 ± 0.08 | 0.095 ± 0.004 | 0 | 0.01 ± 0.02 | 0.34 ± 0.04 | n.m. | |
BJH38 | 37°C PcL,MM | 0.69 ± 0.01 | 7.64 ± 0.71 | 0.093 ± 0.006 | 0 | 0.02 ± 0.01 | 0.06 ± 0.01 | n.m. |
ita32 | 30°C PcL,MM | 0.34 ± 0.03 | 4.74 ± 0.38 | 0.074 ± 0.010 | 0.01 ± 0.01 | 0.17 ± 0.06 | 0.06 ± 0.01 | 0 ± 0 |
37°C PcL,MM | 0.52 ± 0.05 | 8.53 ± 0.43 | 0.062 ± 0.01 | 0.00 ± 0.00 | 0.30 ± 0.01 | 0.01 ± 0.01 | 0 ± 0 | |
BJH58 | 37°C PcL,MM | 0.14 ± 0.02 | n.m. | n.m. | n.m. | n.m. | n.m. | n.m. |
ita35 | 30°C PcL,MM | 0.11 ± 0.01 | 3.26 ± 0.80 | 0.034 ± 0.001 | 0.66 ± 0.07 | 0.00 ± 0.00 | 0.08 ± 0.01 | 0.17 ± 0.07 |
37°C PcL,MM | 0.50 ± 0.03 | 8.98 ± 0.62 | 0.060 ± 0.005 | 0 | 0.37 ± 0.13 | 0.01 ± 0.01 | 0 | |
ita36A | 30°C PcL,MM | 0.12 ± 0.02 | 3.25 ± 0.70 | 0.034 ± 0.000 | 0.66 ± 0.05 | 0.00 ± 0.00 | 0.06 ± 0.02 | 0.17 ± 0.04 |
37°C PcL,MM | 0.46 ± 0.12 | 7.05 ± 0.31 | 0.067 ± 0.031 | 0 ± 0 | 0.21 ± 0.16 | 0.01 ± 0.01 | 0 ± 0 | |
37°C PcMM | 0.53 ± 0.07 | 6.86 ± 0.06 | 0.078 ± 0.011 | n.m. | 0.11 ± 0.12 | 0.08 ± 0.01 | n.m. | |
30°C PcMM | 0.14 ± 0.01 | 3.82 ± 0.12 | 0.034 ± 0.00 | 0.62 ± 0.03 | 0 ± 0 | 0.10 ± 0.03 | 0.2 ± 0.03 | |
28°C PcMM | 0.12 ± 0.00 | 3.73 ± 0.03 | 0.031 ± 0.00 | 0.69 ± 0.01 | n.m. | 0.10 ± 0.01 | 0.17 ± 0.01 |
PcL,MM: two precultures were used, one in LB0, followed by one in MM; PcMM: only one preculture in MM was used; n.m., not measured.