. 2017 Oct 6;115(1):156–164. doi: 10.1002/bit.26446

Table 3.

Shake flask cultivations at high (37°C) and low (28°C/30°C) temperatures

					Yield (mol/mol [glucose])
Strain	Temperature/preculture condition	μ [hr⁻¹]	r _glucose [mmol/gDW/hr]	Y _{biomass/glucose} [g/mmol]	Itaconate	Glutamate	Acetate	Pyruvate
MG1655	30°C Pc^L,MM	0.36 ± 0.01	4.36 ± 0.27	0.082 ± 0.003	0	0.00 ± 0.00	0.34 ± 0.00	0 ± 0
	37°C Pc^L,MM	0.71 ± 0.01	7.62 ± 0.08	0.095 ± 0.004	0	0.01 ± 0.02	0.34 ± 0.04	n.m.
BJH38	37°C Pc^L,MM	0.69 ± 0.01	7.64 ± 0.71	0.093 ± 0.006	0	0.02 ± 0.01	0.06 ± 0.01	n.m.
ita32	30°C Pc^L,MM	0.34 ± 0.03	4.74 ± 0.38	0.074 ± 0.010	0.01 ± 0.01	0.17 ± 0.06	0.06 ± 0.01	0 ± 0
	37°C Pc^L,MM	0.52 ± 0.05	8.53 ± 0.43	0.062 ± 0.01	0.00 ± 0.00	0.30 ± 0.01	0.01 ± 0.01	0 ± 0
BJH58	37°C Pc^L,MM	0.14 ± 0.02	n.m.	n.m.	n.m.	n.m.	n.m.	n.m.
ita35	30°C Pc^L,MM	0.11 ± 0.01	3.26 ± 0.80	0.034 ± 0.001	0.66 ± 0.07	0.00 ± 0.00	0.08 ± 0.01	0.17 ± 0.07
	37°C Pc^L,MM	0.50 ± 0.03	8.98 ± 0.62	0.060 ± 0.005	0	0.37 ± 0.13	0.01 ± 0.01	0
ita36A	30°C Pc^L,MM	0.12 ± 0.02	3.25 ± 0.70	0.034 ± 0.000	0.66 ± 0.05	0.00 ± 0.00	0.06 ± 0.02	0.17 ± 0.04
	37°C Pc^L,MM	0.46 ± 0.12	7.05 ± 0.31	0.067 ± 0.031	0 ± 0	0.21 ± 0.16	0.01 ± 0.01	0 ± 0
	37°C Pc^MM	0.53 ± 0.07	6.86 ± 0.06	0.078 ± 0.011	n.m.	0.11 ± 0.12	0.08 ± 0.01	n.m.
	30°C Pc^MM	0.14 ± 0.01	3.82 ± 0.12	0.034 ± 0.00	0.62 ± 0.03	0 ± 0	0.10 ± 0.03	0.2 ± 0.03
	28°C Pc^MM	0.12 ± 0.00	3.73 ± 0.03	0.031 ± 0.00	0.69 ± 0.01	n.m.	0.10 ± 0.01	0.17 ± 0.01

Pc^L,MM: two precultures were used, one in LB₀, followed by one in MM; Pc^MM: only one preculture in MM was used; n.m., not measured.