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. 2017 Dec 11;48:87. doi: 10.1186/s13567-017-0492-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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SDS-PAGE and Western blot analysis of purified WSSV virions. A Equal amounts of purified WSSV-CN03 and WSSVCN04 virions were lysed, separated on 12% SDS-PAGE gel, and stained with coomassie brilliant blue. B For Western blotting, proteins separated on SDS-PAGE gels were transferred to PVDF membranes. The membranes were probed with indicated primary antibodies, and then incubated with alkaline phosphatase-conjugated goat anti-mouse IgG. The signals were detected using the NBT/BCIP substrate.