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. 2017 Dec 11;48:87. doi: 10.1186/s13567-017-0492-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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qPCR analysis of the infection of WSSV-CN03 and -CN04 in juvenile L. vannamei. L. vannamei were randomly divided into three groups. Each shrimp was fed with 2 mg WSSV-CN03-infected tissue or WSSV-CN04-infected tissue. The shrimp in the control group were fed with WSSV-free tissue. For each group, at 4 hpi and 24 hpi, 5 shrimp were randomly selected for q-PCR analysis. The columns represent the log base 10 of the mean WSSV copy numbers of 5 shrimp in each group, and the standard deviations were calculated. The data were subjected to independent-sample t test as indicated in figure. *P < 0.05; ***P < 0.001. The experiment was repeated three times and representative results from one experiment are shown.