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. 2017 Dec 11;48:87. doi: 10.1186/s13567-017-0492-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunofluorescence analysis of WSSV-CN03 and -CN04 infection in juvenile Litopenaeus vannamei . L. vannamei were randomly divided into three groups. Each shrimp was fed with 2 mg WSSV-CN03-infected tissue or WSSV-CN04-infected tissue. The shrimp in the control group were fed with WSSV-free tissue. At 24 hpi, 5 shrimp were randomly selected from each group for cryosectioning. The slices were probed with anti-VP28 antibody, and the nucleus was stained with DAPI. Bar 20 μm. The experiment was repeated three times and typical results of one experiment are shown.