Fig. 3.

Macroscopic images and microscopic images of histological sections. Images of PD sheets (a–g) and TKA sheets (h–n). a, h Representative images of a PD sheet and TKA sheet attached to a white PVDF membrane. Histological staining for Safranin O (b, i) and toluidine blue (c, j) showed either weak or no staining for all donors (×20). Immunostaining for COL1 (red; d, k), COL2 (green; e, l), ACAN (red; f, m), and FN (green; g, n) showed positive staining for COL1, ACAN, and FN but negative staining for COL2 (×20). (Scale bar = 100 μm)