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. 2017 Nov 11;82(5):813–827. doi: 10.1002/ana.25085

Figure 7.

Figure 7

LPC C26:0 treatment caused neuronal damage in the coculture system with Abcd1–/– microglia. (A) Representative images show cocultured differentiated neuron with wild‐type and Abcd1–/– microglia under normal condition. (B) ImageJ (NIH, Bethesda, MD) quantification of Tuj1 (neuron) and IBA1 (microglia) after coculture of differentiated neurons and primary microglia for 4 days. (C,D) Effect of 15 μM of LPC C26:0 on Tuj1 expression of differentiated ReN cells in the coculture system with primary Abcd1–/– microglia and the impact of MFGE8 antibody supplementation. Bar = 50 µm. (E) Representative image shows microglia (green) phagocytosing neurons (red) and (F) degenerated axons within coculture system with LPC C26:0 supplementation (white arrow). Bar = 50 µm. (G) Quantification of degenerated axons in the coculture system. Data are expressed as mean ± SEM; *p < 0.05. IBA1 = ionizing calcium‐binding adaptor molecule 1; SEM = standard error of the mean; Tuj1 = beta‐III‐tubulin; WT = wild type.