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. 2017 Dec 7;8:2085. doi: 10.3389/fpls.2017.02085

FIGURE 4.

FIGURE 4

Analyses of the enzymatic products and the enzymatic kinetic parameter of the recombinant UGT716A1 protein with flavanol gallates and gallic acids as substrates. (A–F) HPLC chromatograms of enzymatic products from enzymatic assays of recombinant UGT716A1 protein (upper panels) and control (lower panels) with various substrates. (A), catechin gallate; (B), epicatechin gallate; (C), gallocatechin gallate; (D), epigallocatechin gallate; (E), gallic acid; (F), methyl gallic acid. Arrowheads indicate the enzymatic products with the corresponding substrates at a concentration of 100 μM. (G) Proposed schematic structures of glucosides of flavanol gallates. (H) The kinetic parameter of UGT716A1 protein with flavanol gallates and (methyl) gallic acids as substrates and UDP-glucose as sugar donor. Values show the means of analytical triplicates.