Table 1.
Authors | Year | Cell source | Enzyme digestion | Selection | Differentiation | Result |
---|---|---|---|---|---|---|
Shi et al. [24] | 2003 | Human DPSCs | 3 mg/ml collagenase type I, 4 mg/ml dispase | STRO-1+ (MACS) | Odontogenic/osteogenic cells | Production of osteodentin-like structures and fibrous connective tissues |
Laino et al. [27] | 2006 | Human DPSCs/SHEDs | 3 mg/ml collagenase type I, 4 mg/ml dispase | c-Kit+CD34+STRO-1+CD45− (FACS) | Osteogenic cells | High positivity for CD44, RUNX2, and osteocalcin |
Iohara et al. [31] | 2006 | Human, bovine, canine, and porcine DPSCs | – | Hoechst 33342 (FACS) | Odontogenic, chondrogenic, adipogenic, and neurogenic cells | SP cells are enriched for stem cell properties and useful for cell therapy with BMP2 to regenerate dentin |
Yang et al. [25] | 2007 | Rat DPSCs | – | STRO-1+ (FACS) | Odontogenic, neurogenic, adipogenic, myogenic, and chondrogenic cells | STRO-1 selection obtains a more homogeneous cell population with a multilineage differentiation capacity |
Honda et al. [30] | 2007 | Human DPSCs | – | Hoechst 33342 (FACS) | Odontogenic cells | SP cells expressing ABCG2 in human adult dental pulp that differentiate into odontoblast-like cells |
Waddington et al. [39] | 2009 | Rat DPSCs | 4 mg/ml collagenase, 4 mg/ml dispase | LNGFR (MACS) | Osteogenic, adipogenic, \and chondrogenic cells | LNGFR+ DPSCs express CD105 and Notch 2 |
Ricco et al. [87] | 2010 | Human DPSCs | 3 mg/ml collagenase type I, 4 mg/ml dispase | CD34+c-Kit+STRO-1+ (MACS) | Osteogenic cells | CD34+c-Kit+STRO-1+ DPSCs produce mineralized matrix in 2D and 3D cultures |
Iohara et al. [33] | 2011 | Dog DPSCs | – | CD105 (FACS) | Odontogenic/osteogenic, adipogenic, angiogenic, and neurogenic cells | Transplantation of CD105+ DPSCs with SDF-1 completely regenerates dental pulp in a pulpectomy model |
Mikami et al. [38] | 2011 | Human SHEDs | 2 mg/ml collagenase type I | CD271+CD90+CD44 (FACS) | Osteogenic, adipogenic, chondrogenic, and myogenic cells | LNGFR positivity inhibits the differentiation of DPSCs into osteogenic, adipogenic, chondrogenic, and myogenic lineages |
Hosoya et al. [58] | 2012 | Rat DPSCs | 2 mg/ml collagenase, 0.25% trypsin | THY-1High+ (FACS) | Osteogenic cells | Hard tissue formation upon subcutaneous transplantation of THY-1+ cells |
Kawanabe et al. [37] | 2015 | Human SHEDs | 5 mg/ml collagenase type II, 2.5 mg/ml dispase I | SSEA-4+ (FACS) | Osteogenic, adipogenic, and chondrogenic cells | SSEA-4+ SHEDs have a multilineage potential. |
Yasui et al. [50] | 2016 | Human DPSCs | 2 mg/ml collagenase, 4 mg/ml dispase | LNGFRLow+THY-1High+ (FACS) | Osteogenic, adipogenic, and chondrogenic cells | LNGFRLow+THY-1High+ DPSCs promote osteogenic differentiation. |
BMP2 bone morphogenetic protein 2, FACS fluorescence-activated cell sorting, LNGFR low-affinity nerve growth factor, MACS magnetic-activated cell sorting, SDF-1 stromal cell-derived factor-1, SP side population, SSEA-4 stage-specific embryonic antigen-4, THY-1 thymocyte antigen 1