Fig. 4.

Automated multi-parametric image analysis of sprouting angiogenesis with pericytes. a Confocal micrograph (maximum intensity projection) showing a bead with sprouts stained for F-actin (green), nuclei (blue), endothelial nuclei (white), and pericytes (red). b–d Result images showing segmentation results (white): segmented bead (b), total sprout area (c) and pericyte area (d). e Cell classification result showing endothelial nuclei (yellow) and pericyte nuclei (magenta). f–g Result images from the cell classification showing EC nuclei (f) and pericyte nuclei (g). h Sprout skeleton for length measurements. i–l Magnified details of the marked region in (a, f–h). m Result table shown after running our plugin on the example image shown in a. Scale bars, a–h 200 μm; i–l 100 μm