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. 2017 Dec 11;17:529. doi: 10.1186/s12906-017-2043-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Generation and characterization of HFF-derived hiPSCs. a Typical morphology of human foreskin fibroblast (HFFs). Phase contrast, AP staining and immunostaining of the pluripotent-associated marker Oct4; Counter-staining with DAPI. b RT-PCR results demonstrated that three randomly selected 2F HFF-iPSC colonies expressed the pluripotent marker genes KIf4, c-Myc, Oct4, Nanog, and Sox2. c FASC analysis of the colonies differentiated into HFF-derived iPSCs in the present of SGT-4 on day 9 after transfection hiPSC markers including SSEA1, SSEA3, and SSEA4 antibodies