Fig. 3.

miR-124 directly targeted 3′UTR of DLL4 in NSCs. a Online TargetScan website predicted the putative binding sequences of miR-124 in the 3′UTR of DLL4. The sequences of mutated DLL4-3′UTR were also shown. b NSCs were cotransfected with the constructed luciferase reporter plasmids (DLL4-3′UTR-WT or DLL4 3′UTR-MUT) and miR-124 or miR-control, and then luciferase reporter assay was performed to detect luciferase activity. The expression of DLL4 at mRNA (c) and protein (d) levels were assessed by qRT-PCR and western blot in NSC cells transfected with miR-124, anti-miR-124, or matched controls. *P < 0.05, n = 3