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. 2017 Sep 6;2(9):5529–5537. doi: 10.1021/acsomega.7b00769

Figure 5.

Figure 5

Analysis of the activation of rMZ-II and rP2-II. rMZ-II (1.4 μM, panel A) and rP2-II (1.4 μM, panel B) were incubated in different mixtures with PCPS vesicles (20 μM), DAPA (3 μM), and rhfVaWT (20 nM) or rhfVaNR→DE (20 nM). The reaction was started by the addition of fXa, and the samples were treated as detailed in the Experimental Section. Lanes 1–9 represent samples of the reaction mixture following incubation of prothrombinase assembled with rhfVaWT with rMZ-II or rP2-II before (lane 1) or following 1, 3, 5, 10, 20, 45, 60, and 120 min of incubation with fXa, respectively. Lanes 10–18 represent samples of the reaction mixture following incubation of prothrombinase assembled with rhfVaNR→DE with rMZ-II or rP2-II before (lane 10) or following 1, 3, 5, 10, 20, 45, 60, and 120 min of incubation with fXa, respectively. Positions of hPro-derived fragments are indicated to the right, as detailed in the legend of Figure 3. For easy reading of the article, the rhfVa species used for the reconstitution of prothrombinase are also shown.