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. 2017 Sep 6;2(9):5529–5537. doi: 10.1021/acsomega.7b00769

Figure 7.

Figure 7

Gel electrophoresis analyses for cleavage of FPR-MzT. FPR-MzT (1.4 μM) was incubated in different mixtures with PCPS vesicles (20 μM) and rhfVa as described in the legend to Figure 3. The reactions were started by the addition of fXa, and the samples were further treated, scanned, and quantified as detailed in the Experimental Section. Panel A, prothrombinase assembled with rhfVaWT; panel B, prothrombinase assembled with rhfVaNR→DE; M represents the lane with the molecular weight markers (from top to bottom): Mr 50 000, Mr 36 000, Mr 22 000. Lanes 1–17 represent samples from the reaction mixture before (0 min) the addition of fXa and 20, 40, 60, 80, 100, 120, 140, 160, 180, 200, 220, and 240 s, 5, 6, 10, and 20 min, respectively, following the addition of fXa. The hPro-derived fragments are shown as detailed in the legend to Figure 3. The recombinant rhfVa species used for the reconstitution of prothrombinase is also shown under each panel.