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. 2017 Nov 6;37:24. doi: 10.1186/s41232-017-0054-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effects of abatacept on the mRNA expression for CD80 and CD86 of SEB-stimulated monocytes. Highly purified monocytes (1 × 10⁶/well) from healthy individuals were cultured in the presence of SEB (100 pg/ml) in each well of 24-well flat-bottomed microtiter plates with abatacept (100 μg/ml) or control IgG-Fc (100 μg/ml). After 24 h of incubation, the cells were harvested and the expression of mRNA for CD80 and CD86 were examined by quantitative RT-PCR. Data are expressed as the ratio to copy numbers of β-actin mRNA. Statistical significance was evaluated by Wilcoxon’s signed-rank test. CD80 (a) and CD86 (b)