Fig. 2.

Extracellular antibody binding is critical for the effect on α-syn oligomer accumulation. Cells were exposed to Cy3-α-syn oligomers and mAb47 in three different combinations. a The cells were either exposed to α-syn oligomers for 24 h (α-syn), resulting in high intracellular accumulation, or to mAb47:oligomer complex (α-syn+47). A clear reduction in α-syn fluorescence signal was detected. Parallel cultures received mAb47 24 h prior to α-syn oligomer exposure (α-syn+47 pre), resulting in a minor reduction of the fluorescence signal. When mAb47 was added 24 h after the oligomer exposure (α-syn+47 post), there was no effect on α-syn accumulation. b Fluorescence quantification of Cy3-α-syn area and c intensity demonstrated that there was only a significant decrease of α-syn accumulation when mAb47 was added at the same time as the oligomers (α-syn+47) or added before the α-syn exposure (α-syn+47 pre). Scale bars = 10 μm. Data are presented as mean ± SD from three independent experiments and levels of significance were set to *P < 0.05, **P < 0.01, and ***P < 0.001