Fig. 3.

Treatment with irrelevant antibodies has no effect on α-syn accumulation. To investigate the specificity of the antibody-mediated reduction of α-syn accumulation, cells were exposed to Cy3-oligomers pre-incubated with an irrelevant antibody of the same IgG subclass as mAb47 (IgG1). The IgG1 LY-128 has no affinity for α-syn and therefore served as an irrelevant isotype control. Alpha-synuclein oligomers were pre-incubated with mAb47 or LY-128 prior to the 24-h cell exposure. a The cultures that were treated with mAb47 (α-syn+47) displayed a reduced α-syn accumulation. This effect was significantly stronger than in cultures treated with LY-128 (α-syn+LY128). b Quantification of Cy3-α-syn fluorescence area and (c) intensity confirmed a significant decrease of α-syn signal in mAb47-treated cultures compared to LY-128-treated cultures. Scale bars = 10 μm. Data are presented as mean ± SD from three independent experiments and levels of significance were set to *P < 0.05, **P < 0.01, and ***P < 0.001