Fig. 6.

Treatment with mAb47 prevents α-syn oligomer-mediated mitochondrial stress effects. During the 24 h exposure to α-syn oligomers or Ab:oligomer complexes, cells were transfected with Cell light Mitochondria-GFP to label the mitochondria. a Astrocytes exposed to α-syn oligomers frequently displayed a disrupted mitochondrial network and mitochondrial swelling. b The astrocytes in cultures exposed to α-syn oligomers pre-incubated with mAb47 displayed an elongated, branched mitochondrial network throughout the cells, similar to that of c non-treated cells. d Cells with disrupted mitochondrial networks were counted and normalized against the number of transfected cells. Oligomer exposure led to a clear increase in cells with mitochondrial fragmentation, whereas cultures exposed to oligomers pre-incubated with mAb47 did not significantly differ from the untreated control cells. Scale bars = 10 μm. Data are presented as mean ± SD from three independent experiments and levels of significance were set to *P < 0.05, **P < 0.01, and ***P < 0.001