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Purified human pericytes and HUVECs co-form a dual-layered microvascular tube in 3D Matrigel plugs. Confocal microscopy revealed the formation of a dual-layer microvascular structure by fluorescent activated cell sorting-purified human pericytes (green) and HUVECs (red) in a 3D Matrigel plug where HUVECs formed an endothelial tube tightly surrounded by pericytes at 72 h after encapsulation: (A) HUVECs, (B) human pericytes, and (C) merge (scale bars: 50 μm).
