Table 1.
Cell surface markers for flow cytometry. Antibody volumes are based on manufacturer’s recommendations to ensure limited inter-assay variability for batch analysis of long-term studies.)
| Laser | Filter | Fluorochrome | Specificity | Clone | Cat. No | Volume (μl) |
|---|---|---|---|---|---|---|
| Blue 488 nm | 530/30 | FITC | CD16 | 3G8 | 555406 | 20 |
| 695/40 | PerCP-Cy™5.5 | HLA-DR | G46-6 | 560652 | 5 | |
|
| ||||||
| Yellow -Green 561 nm | 582/15 | PE | CD56 | B159 | 555516 | 20 |
| 610/20 | PE-CF594 | CD19 | HIB-19 | 562294 | 5 | |
| 780/60 | PE-Cy™7 | CD8 | RPA-T8 | 557746 | 5 | |
|
| ||||||
| Red 640 nm | 670/14 | Alexa Fluor 647 | CD127 | HIL-7R-M21 | 558598 | 20 |
| 730/45 | Alexa Fluor 700 | CD4 | RPA-T4 | 557922 | 5 | |
| 780/60 | APC-Cy™7 | FVS780 | 565388 | 1 | ||
|
| ||||||
| Violet 405 nm | 450/50 | BV421 | CD45RO | UCHL1 | 562641 | 5 |
| 525/50 | BV510 | CD3 | UCHT1 | 563109 | 5 | |
| 605/12 | BV605 | CD14 | M5E2 | 564054 | 5 | |