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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Neuropharmacology. 2017 Oct 16;128:255–268. doi: 10.1016/j.neuropharm.2017.10.014

Figure 4. Confocal microscope images of lamina 1 neurons with and without NK1R internalization.

Figure 4

Images were taken from the L4 spinal segment of the rats used from the experiment in Fig. 3. Rats received three intrathecal injections using the following time line: 0 h - saline or BDNF (0.3 μg); 3 h - saline or DAMGO (3 nmol); 4 h - NMDA + D-Ser (10 nmol each). A. Rat injected with saline-saline-NMDA; there was no NK1R internalization. B. Rat injected with BDNF-saline-NMDA; NK1R internalization is clear in 5 out of 7 cells. C. Rat injected with BDNF-DAMGO-NMDA; there was no NK1R internalization. Images are 10 optical sections taken with a 20× objective (main panels, voxel size 692 × 692 × 854 nm, scale bar 100 μm) or a 63× objective (insets, voxel size 132 × 132 × 377 nm, scale bar 10 μm).